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epithelial cells primary mammary atcc pcs 600 010  (ATCC)


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    ATCC epithelial cells primary mammary atcc pcs 600 010
    Epithelial Cells Primary Mammary Atcc Pcs 600 010, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epithelial+cells+primary+mammary+atcc+pcs+600+010/Primary+Mammary+Epithelial+Cells%3B+Normal%2C+Human/us12583939-738-35-39
    Average 99 stars, based on 1327 article reviews
    epithelial cells primary mammary atcc pcs 600 010 - by Bioz Stars, 2026-10
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    Expressing:

    Article Title: Bispecific antigen binding molecules that bind HER2, and methods of use thereof
    Article Snippet: .. TABLE 7 Cell Lines and Relative HER2 Expression Relative HER2 Cell Line Source Catalog # Expression Primary Myocardial Promo C12811 6% cells Cell Primary Prostate ATCC PCS-440-010 1% Epithelial cells Primary Lung ATCC PCS-300-010 1% Epithelial cells Primary Mammary ATCC PCS-600-010 1% Epithelial cells MDA-MB468 ATCC HTB-132 0% MDA-MB231 ATCC HTB-26 3% HepG2 ATCC HB-8065 13% EBC-1 6% (Lung Carcinoma) MCF7 ATCC HTB-22 15% T47D ATCC HTB-133 24% BT483 ATCC HTB121 37% ZR751 ATCC CRL-1500 39% JIMT1 DSMZ ACC589 52% MDA-MB361 ATCC HTB-27 82% MDA-MB453 ATCC HTB-131 60% SK-BR-3 ATCC HTB-30 100% B1474 ATCC HTB-20 89% N87 (NCI-N87) ATCC HTB-5822 93% Calu-3 ATCC HTB-55 84% SKOV-3 ATCC HTB-77 93% Cells were plated on collagen-coated, black wall, 96-well, optical plates (Greiner, Cat #655936) at 2.5×105 cells per well in their appropriate culture media and incubated overnight at 37° C. in 5% CO2. .. The following day, cell were incubated for 30 minutes with 10 ug/mL of Alexa647 labeled (Thermo, Cat #A37573) antibodies in 50 uL of DMEM at 4° C. After, cells were washed twice with cold DMEM+10% FBS and incubated with Δ % paraformaldehyde (Electron Microcopy Sciences, Cat #15710)+0.075% Saponin (Sigma, Cat #S4521)+10 ug/ml Hoechst (Lifetech, #H3569) in PBS for 10 minutes at room temperature.

    Multiple Displacement Amplification:

    Article Title: Bispecific antigen binding molecules that bind HER2, and methods of use thereof
    Article Snippet: .. TABLE 7 Cell Lines and Relative HER2 Expression Relative HER2 Cell Line Source Catalog # Expression Primary Myocardial Promo C12811 6% cells Cell Primary Prostate ATCC PCS-440-010 1% Epithelial cells Primary Lung ATCC PCS-300-010 1% Epithelial cells Primary Mammary ATCC PCS-600-010 1% Epithelial cells MDA-MB468 ATCC HTB-132 0% MDA-MB231 ATCC HTB-26 3% HepG2 ATCC HB-8065 13% EBC-1 6% (Lung Carcinoma) MCF7 ATCC HTB-22 15% T47D ATCC HTB-133 24% BT483 ATCC HTB121 37% ZR751 ATCC CRL-1500 39% JIMT1 DSMZ ACC589 52% MDA-MB361 ATCC HTB-27 82% MDA-MB453 ATCC HTB-131 60% SK-BR-3 ATCC HTB-30 100% B1474 ATCC HTB-20 89% N87 (NCI-N87) ATCC HTB-5822 93% Calu-3 ATCC HTB-55 84% SKOV-3 ATCC HTB-77 93% Cells were plated on collagen-coated, black wall, 96-well, optical plates (Greiner, Cat #655936) at 2.5×105 cells per well in their appropriate culture media and incubated overnight at 37° C. in 5% CO2. .. The following day, cell were incubated for 30 minutes with 10 ug/mL of Alexa647 labeled (Thermo, Cat #A37573) antibodies in 50 uL of DMEM at 4° C. After, cells were washed twice with cold DMEM+10% FBS and incubated with Δ % paraformaldehyde (Electron Microcopy Sciences, Cat #15710)+0.075% Saponin (Sigma, Cat #S4521)+10 ug/ml Hoechst (Lifetech, #H3569) in PBS for 10 minutes at room temperature.

    Incubation:

    Article Title: Bispecific antigen binding molecules that bind HER2, and methods of use thereof
    Article Snippet: .. TABLE 7 Cell Lines and Relative HER2 Expression Relative HER2 Cell Line Source Catalog # Expression Primary Myocardial Promo C12811 6% cells Cell Primary Prostate ATCC PCS-440-010 1% Epithelial cells Primary Lung ATCC PCS-300-010 1% Epithelial cells Primary Mammary ATCC PCS-600-010 1% Epithelial cells MDA-MB468 ATCC HTB-132 0% MDA-MB231 ATCC HTB-26 3% HepG2 ATCC HB-8065 13% EBC-1 6% (Lung Carcinoma) MCF7 ATCC HTB-22 15% T47D ATCC HTB-133 24% BT483 ATCC HTB121 37% ZR751 ATCC CRL-1500 39% JIMT1 DSMZ ACC589 52% MDA-MB361 ATCC HTB-27 82% MDA-MB453 ATCC HTB-131 60% SK-BR-3 ATCC HTB-30 100% B1474 ATCC HTB-20 89% N87 (NCI-N87) ATCC HTB-5822 93% Calu-3 ATCC HTB-55 84% SKOV-3 ATCC HTB-77 93% Cells were plated on collagen-coated, black wall, 96-well, optical plates (Greiner, Cat #655936) at 2.5×105 cells per well in their appropriate culture media and incubated overnight at 37° C. in 5% CO2. .. The following day, cell were incubated for 30 minutes with 10 ug/mL of Alexa647 labeled (Thermo, Cat #A37573) antibodies in 50 uL of DMEM at 4° C. After, cells were washed twice with cold DMEM+10% FBS and incubated with Δ % paraformaldehyde (Electron Microcopy Sciences, Cat #15710)+0.075% Saponin (Sigma, Cat #S4521)+10 ug/ml Hoechst (Lifetech, #H3569) in PBS for 10 minutes at room temperature.



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    Image Search Results


    The cell viability of ARPE-19 human retinal epithelial cells treated with fruit (up panel) and leaf (down panel) extracts at varying concentrations for 24 h and 48 h. Extracts were obtained from olives grown at different distances from the Yatağan TPP, Deştin Village (farthest location), Central (middle location), and Thermal (Şahinler Village - closest location). Values represent mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: Thermal power plant proximity alters Olive composition and induces cytotoxicity in human cells

    doi: 10.1038/s41598-025-18066-y

    Figure Lengend Snippet: The cell viability of ARPE-19 human retinal epithelial cells treated with fruit (up panel) and leaf (down panel) extracts at varying concentrations for 24 h and 48 h. Extracts were obtained from olives grown at different distances from the Yatağan TPP, Deştin Village (farthest location), Central (middle location), and Thermal (Şahinler Village - closest location). Values represent mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The cells included MCF10A (ATCC, CRL-10317TM) human mammary gland epithelial cells, ARPE-19 (ATCC, CRL-2302TM) human eye retinal pigment epithelial cells, HUVEC (ATCC, CRL-1730TM) primary human umbilical vein endothelial cells, and BEAS-2B (ATCC, CRL-3588TM) epithelial cells isolated from normal human bronchial epithelium.

    Techniques:

    The cell viability of MCF10A human breast epithelial cells treated with fruit (up panel) and leaf (down panel) extracts at varying concentrations for 24 h and 48 h. Extracts were obtained from olives grown at different distances from the Yatağan TPP, Deştin Village (farthest location), Central (middle location), and Thermal (Şahinler Village - closest location). Values represent mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: Thermal power plant proximity alters Olive composition and induces cytotoxicity in human cells

    doi: 10.1038/s41598-025-18066-y

    Figure Lengend Snippet: The cell viability of MCF10A human breast epithelial cells treated with fruit (up panel) and leaf (down panel) extracts at varying concentrations for 24 h and 48 h. Extracts were obtained from olives grown at different distances from the Yatağan TPP, Deştin Village (farthest location), Central (middle location), and Thermal (Şahinler Village - closest location). Values represent mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The cells included MCF10A (ATCC, CRL-10317TM) human mammary gland epithelial cells, ARPE-19 (ATCC, CRL-2302TM) human eye retinal pigment epithelial cells, HUVEC (ATCC, CRL-1730TM) primary human umbilical vein endothelial cells, and BEAS-2B (ATCC, CRL-3588TM) epithelial cells isolated from normal human bronchial epithelium.

    Techniques:

    The cell viability of BEAS-2B human bronchial epithelial cells treated with fruit (up panel) and leaf (down panel) extracts at varying concentrations for 24 h and 48 h. Extracts were obtained from olives grown at different distances from the Yatağan TPP, Deştin Village (farthest location), Central (middle location), and Thermal (Şahinler Village - closest location). Representative cells after the MTT assay are given after leaf extracts for 48 h (images taken by 5x objective of Zeiss AxioVert inverted microscope). Values represent mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: Thermal power plant proximity alters Olive composition and induces cytotoxicity in human cells

    doi: 10.1038/s41598-025-18066-y

    Figure Lengend Snippet: The cell viability of BEAS-2B human bronchial epithelial cells treated with fruit (up panel) and leaf (down panel) extracts at varying concentrations for 24 h and 48 h. Extracts were obtained from olives grown at different distances from the Yatağan TPP, Deştin Village (farthest location), Central (middle location), and Thermal (Şahinler Village - closest location). Representative cells after the MTT assay are given after leaf extracts for 48 h (images taken by 5x objective of Zeiss AxioVert inverted microscope). Values represent mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The cells included MCF10A (ATCC, CRL-10317TM) human mammary gland epithelial cells, ARPE-19 (ATCC, CRL-2302TM) human eye retinal pigment epithelial cells, HUVEC (ATCC, CRL-1730TM) primary human umbilical vein endothelial cells, and BEAS-2B (ATCC, CRL-3588TM) epithelial cells isolated from normal human bronchial epithelium.

    Techniques: MTT Assay, Inverted Microscopy

    Basic spatial transcriptomic data visualization (A) Number of unique molecular identifiers (UMIs) per capture spot. Raw data, 8 μm binning. (B) Spatial distribution of UMIs overlayed on the H&E image. Raw data, 8 μm binning, cropped to display values between 0 and 60. (C) Sample annotation across the capture area. (D) Cell type annotation facilitated by corresponding fluorescence image of cells that were transduced with unique reporters. H2B-iRFP+ human primary mammary fibroblasts, EF1-tdTomato+ MCF10A mammary epithelial cells, and H2B-mVenus+ MDA-MB-231 breast cancer cell line. Scale bar = 500 μm.

    Journal: STAR Protocols

    Article Title: Protocol for single-cell spatial transcriptomic profiling of cultured cells and engineered tissues without embedding or sectioning

    doi: 10.1016/j.xpro.2025.104074

    Figure Lengend Snippet: Basic spatial transcriptomic data visualization (A) Number of unique molecular identifiers (UMIs) per capture spot. Raw data, 8 μm binning. (B) Spatial distribution of UMIs overlayed on the H&E image. Raw data, 8 μm binning, cropped to display values between 0 and 60. (C) Sample annotation across the capture area. (D) Cell type annotation facilitated by corresponding fluorescence image of cells that were transduced with unique reporters. H2B-iRFP+ human primary mammary fibroblasts, EF1-tdTomato+ MCF10A mammary epithelial cells, and H2B-mVenus+ MDA-MB-231 breast cancer cell line. Scale bar = 500 μm.

    Article Snippet: Primary mammary epithelial cells , ATCC , PCS-600-010.

    Techniques: Fluorescence, Transduction